Molecular characterization of an Akabane virus isolate from West Java, Indonesia
نویسندگان
چکیده
We isolated an arbovirus from bovine blood in Indonesia. The arbovirus was obtained from the plasma of a cow showing no clinical symptoms in West Java in February 2014, and was identified as Akabane virus (AKAV) by AKAV-specific RT-PCR and subsequent sequence analysis. Phylogenetic analysis based on partial S segment indicated the AKAV isolate, WJ-1SA/P/2014, was most closely related with two isolates from Israel and Turkey reported in 2001 and 2015, respectively, and that WJ-1SA/P/2014 isolate belongs to AKAV genogroup Ib. This is the first isolation of AKAV from Indonesia.
منابع مشابه
Effects of insulin-like growth factor-induced Wharton jelly mesenchymal stem cells toward chondrogenesis in an osteoarthritis model
Objective(s): This study aimed to determine the collagen type II (COL2) and SOX9 expression in interleukin growth factor (IGF-1)-induced Wharton’s Jelly mesenchymal stem cells (WJMSCs) and the level of chondrogenic markers in co-culture IGF1-WJMSCs and IL1β-CHON002 as osteoarthritis (OA) cells model. Materials and Methods: WJMSCs were induced with IGF1 (75, 150, and 300 ng/ml) to enhance their ...
متن کاملA history of Floral diversity (pollen, spores and algal) during the latest Holocene in the Bandung basin based on palynological analysis in Cihideung, West Java, Indonesia
Floral diversity is a measure of number of type flora in an area, and reflects how vegetation develops in response to the environmental condition during a certain time interval. The present study aims to examine changes in the diversity of vegetation (pollen, spores and algae), evenness, and similarity in the Bandung Basin through a core of 240 cm depth using a ground drill, as well as the ...
متن کاملMolecular characterization and construction of an infectious clone of a pepper isolate of Beet curly top Iran virus
Geminiviruses cause curly top disease, in dicotyledonous plants which constrains host crop production. Beet curly top Iran virus (BCTIV) is a widespread Becurtovirus (family Geminiviridae) in numerous areas within Iran. In this study, we isolated and analyzed a full-length genomic DNA of a new variant of BCTIV from pepper crops in the Kaftark region, east of Shiraz (proposed acronym: BCTIV-Kaf ...
متن کاملMangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis
Objective(s): This study aims to evaluate the activity of mangosteen peels extract (MPE) as protection agent on induced-glucose mesangial cells (SV40 MES 13 cell line (Glomerular Mesangial Kidney, Mus Musculus)). Materials and Methods: MPE was performed based on maceration method. Cytotoxic assay was performed based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophen...
متن کاملSerological and Molecular Detection of Akabane Virus in Iran
The present study was conducted on sixty healthy female domestic ruminants that suffered from still births or abortion, to find sero-positive cases and also to determine the probable role of uterine tissue in diagnosis of akabane viral infection and viral persistency. Because of this, a serological test (ELISA) and molecular (RT-PCR) diagnostic methods were used to find the seropositive cases a...
متن کامل